Journal: bioRxiv

Article Title: Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection

doi: 10.1101/2023.01.25.525624

Figure Lengend Snippet: (A) Images represent calcofluor white (CFW) staining of WT CEA10, Δ zfpA , and OE:: zfpA following overnight exposure to 1 μg/mL caspofungin (CSP) or DMSO. CFW staining is represented by cyan and cytoplasmic RFP signal is shown in magenta. Scale bar = 50 μm. (B) Experimental setup for chitin stimulation with CaCl 2 /CFW. Spores were incubated for 8 h at 37°C or until germination in liquid GMM or liquid GMM supplemented with 0.2 M CaCl 2 and 100 μg/mL CFW. After germination, media was replaced for GMM + 1 μg/mL caspofungin or DMSO and hyphae were incubated for an additional 12 h before detecting PrestoBlue viability reagent signal in a plate reader. (C) Bars represent mean±s.d. of relative fungal viability following caspofungin exposure. Relative viability was calculated by normalizing the mean signal of caspofungin-treated wells to the mean signal of DMSO-treated wells. All experiments included 5 wells/condition. Data are pooled from 3 independent experiments. p values calculated by ANOVA with Sidak’s multiple comparisons. ** p <0.01,*** p <0.001.

Article Snippet: Comparison of fungal viability in was done using ANOVA with Sidak’s multiple comparisons (GraphPad Prism version 9).

Techniques: Staining, Incubation